Animal Models of middle cerebral artery occlusion - 循环系统和脑血管疾病模型

循环系统和脑血管疾病模型目录

Animal Models of middle cerebral artery occlusion

ØIntroduction

Focal cerebral ischemia, mimics occlusion of a cerebral artery either

by an embolus or local thrombosis, with middle cerebral artery occlusion (MCAO) being the most frequent, making it necessary to establish stable animal models and develop new therapeutic strategies to alleviate ischemic damage.

ØMethod

MCAO models can be subdivided into two groups: transient MCAO (tMCAO) and permanent MCAO(pMCAO). In tMCAO, the occlusion of the artery is sustained for a short period (between 30-120 min) and reperfusion is allowed after. In pMCAO models, the occlusion of MCA is maintained until animal sacrificed. The tMCAO mimics a therapeutic intervention (reperfusion) in stroke patients, while pMCAO does not reproduce a therapeutic intervention.

ØEndpoint

Behavioral evaluation: Longa score, modified Neurological Severity Score.

Cerebral blood flow monitoring: Laser speckle imaging(LSI).

Histochemical stain: TTC staining, Nissl's staining, etc.

MCAO model in SD rats induced by suture method